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For staining, cells were permeabilised with 0.5% Triton X-100 for 5mins in room temperature (RT) and blocked in 3% BSA/5% FCS/PBS for 1h

When similar types of stimulation are applied prior to paired associative stimulation (PAS) with a 25-ms interval, which is a protocol to induce long-term potentiation-like plasticity in M1, PAS can be bidirectionally modulated: cTBS cerebellum and cathodal tDCS cerebellum lead to significant enhancement of PAS-induced M1 plastic effect above the ShamTBS cerebellum +PAS M1 level, while iTBS cerebellum and anodal tDCS cerebellum lead to its abolition [247, 248]

Linards et al., 2022

This strategy is used for PEG-Loxenatide from Haosen Pharmaceutical